However, these programs usually do not provide an adequate solution for the processing of these raw data (coming from one or multiple runs) into meaningful results, such as normalized and calibrated relative quantities. The software programs provided along with the various qPCR instruments allow for straightforward extraction of quantification cycle values from the recorded fluorescence measurements, and at best, interpolation of unknown quantities using a standard curve of serially diluted known quantities. The ease of use and high sensitivity, specificity and accuracy has resulted in a rapidly expanding number of applications with increasing throughput of samples to be analyzed. Since its introduction more than 10 years ago, quantitative PCR (qPCR) has become the standard method for quantification of nucleic acid sequences.
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